Like other cities at the time, Twinsburg finds itself amidst the beginning of the Civil War.
There were 120 documented volunteers from Twinsburg who enlisted with various regiments, including:
- 1st Regimental Iowa Volunteer Infantry
 - 1st Regimental Michigan Volunteer Infantry
 - 1st Regiment, O.V.I.
 - 2nd Regiment, O.V.I.
 - 6th Ohio Volunteer Cavalry
 - 6th Regiment, O.V.I.
 - 7th Regiment, O.V.I.
 - 7th U.S Regulars
 - 9th Independent Battery, Ohio Light Artillery
 - 10th Regiment, O.V.I.
 - 13th Regiment, O.V.I.
 - 17th Regiment, O.V.I.
 - 18th Regiment, Wisconsin Volunteer Infantry
 - 19th Regiment, O.V.I.
 - 20th Independent Battery, Ohio Volunteer Light Artillery
 - 21st Regiment, O.V.I.
 - 23rd Regiment, O.V.I.
 - 24th Regiment, Connecticut Volunteer Infantry
 - 30th Regiment, O.V.I.
 - 41st Regiment, O.V.I.
 - 49th Regiment, O.V.I.
 - 84th Regiment, O.V.I.
 - 85th Regiment, O.V.I.
 - 103rd Regiment, O.V.I.
 - 104th Regiment, O.V.I.
 - 105th Regiment, O.V.I.
 - 115th Regiment, O.V.I.
 - 125th Regiment, O.V.I.
 - 150th Regiment, O.V.I.
 - 177th Regiment, O.V.I.
 
Note: O.V.I. is the abbreviation for Ohio Volunteer Infantry.
For further reading on Twinsburg’s soldiers during the Civil War, look for Beyond the Monument: The Civil War Soldiers of Twinsburg, Ohio written by Veronica Hughes, published in 2017.